Book Review: Sean B. Carroll, The Making of the Fittest: DNA and the Ultimate Record of Evolution (NY: W.W. Norton 2006). 301 pp.

by Kenneth W. Krause.

Kenneth W. Krause is a contributing editor and “Science Watch” columnist for the Skeptical Inquirer.  Formerly a contributing editor and books columnist for the Humanist, Kenneth contributes regularly to Skeptic as well.  He may be contacted at krausekc@msn.com.

Publishers have flooded the popular science market in recent years with respectable but frustratingly sketchy accounts of evolution.  Generalizations, metaphors, and defensive polemics have all too frequently been offered not as supplements to, but as surrogates for, authentic science.  Arguably, the most essential and epic narrative ever, notwithstanding those relating to the origins of life and the universe itself, surely deserves better.  And so does the reasonably intelligent reader.

Enter Sean Carroll, University of Wisconsin-Madison professor of genetics, who has served up an intellectual feast for the second time in as many years.  In 2005, he catered Endless Forms Most Beautiful, the much-acclaimed application of embryonic development science to the standard evolutionary paradigm.  And now, in The Making of the Fittest, Carroll shares with us some of the finer and more satisfying details—the meat and potatoes, if you will—of natural selection and descent with modification.  A rapidly expanding library of DNA sequences and the new science of genomics, the comparative study of various species’ DNA, have finally allowed scientists, nearly 150 years following the first publication of Darwin’s On the Origin of Species, to actually see how the fittest are made, to identify the specific genetic changes that have enabled species to adapt to their diverse environments through the ages.

Carroll begins with a look at the most ancient DNA on Earth, genetic text that has somehow withstood a steady barrage of mutations for more than two billion years.  These “immortal genes” endure not because they avoid all mutations, but rather because natural selection has “purified” certain amino acid sequences to prevent them from changing in ways that would compromise certain functions basic to all domains of life, archaea, bacteria, and eukaryotes (including humans) alike.

Carroll’s reasoning goes like this:  Each amino acid is composed of three nucleotide bases, or a triplet (AAG, ACT, etc.), in the DNA molecule.  For each possible triplet, there are 576 potential single random base mutations.  Some changes are called “synonymous” because, despite mutation, the resulting triplets encode the same amino acid, allowing the protein to perform an equivalent function.  Other changes are said to be “nonsynonymous” because mutation results in a different amino acid, which in turn alters the protein’s utility.  If mutation occurred in a completely random fashion, one would expect nonsynonymous changes to outnumber synonymous changes three to one.  Not so.  In fact, the real-world ratio is reversed, three to one in favor of synonymous changes.  “DNA sequences that encode the same protein but that are substantially different,” Carroll surmises, “are unmistakable evidence of natural selection allowing mutations that do not change protein function, while acting to eliminate mutations that would.”

But evolution is not merely loss prohibitive; it is intensively creative as well.  Consider first the development of color vision in certain primates.  All Old World (African and Asian) apes and monkeys, including humans, possess trichromatic color vision encoded by three opsin genes, SWS (sensitive to blue light tuned to a wavelength of 417 nanometers), MWS (green to 530 nm), and LWS (red to 560 nm).  New World monkeys and most other mammals have dichromatic vision and just a single gene responsible for encoding the MWS/LWS opsin (light to wavelengths from 510 to 550 nm).

Carroll demonstrates how, in humans, the first set of three opsins evolved from the second set of two possessed by the common ancestor of apes and Old World monkeys.  Our separate green and red opsins are 98 percent similar and lie together head-to-toe on our X chromosome.  Such characteristics are highly suggestive of gene duplication followed by a divergence of function.  By isolating amino acids, replacing one with another, and measuring the effects of such replacements, biologists have determined that only three amino acid positions are responsible for the fine spectral tuning of human MWS and LWS opsins to 530 nm and 560 nm.  Such precise color tuning, Carroll concludes, must have been under intense selective pressure in the natural world.  Indeed, detailed field studies of food preference and consumption habits among trichromatic chimpanzees, lemurs, and colobus and spider monkeys consistently revealed a fondness for redder leaves, indicative of high protein levels and low toughness.

Discriminating between a light source’s wavelengths, of course, is the occupation of the eye’s cone photoreceptor cells, which are most useful in brightly lit environments.  Consider next the evolution of deep-sea vision.  Seeing in dim light, by contrast, requires the use of a species’ rod photoreceptors.  Rhodopsins in most terrestrial animals are tuned to maximally absorb wavelengths of approximately 500 nm; but, at ocean depths of 200 meters or so, only a narrow band of blue light with a wavelength of 480 nm is available.  Amazingly, the rhodopsins of dolphins, Sowerby’s beaked whales, and deep-sea fish are “blue-shifted,” or fine-tuned 10 to 20 nm toward the blue end of the light spectrum.

Exactly how did this happen?  By replacing amino acids found in one species with those of another, scientists have distinguished three positions, 83, 292, and 299, that are primarily responsible for the 11 nm shift in bottlenose dolphins.  The beaked whale’s rhodopsin is further blue-shifted to 484 nm and differs from its dolphin counterpart only at site 299.  Interestingly, deep-sea eels possess a rhodopsin that is blue-shifted to 482 nm and contains the same three crucial amino acids as that of the beaked whale.  Shallow-water eels, by contrast, have a rhodopsin sensitive to a wavelength of 502 nm, akin to that of terrestrial mammals, and identical at the three crucial sites to the rhodopsins of harbor seals and manatees, two surface-dwelling mammals.  Perhaps even more significant, however, is the fact that eels are fish, the evolutionary lines of which split away from other vertebrates hundreds of millions of years ago.  Whales and dolphins, of course, are cetaceans—mammals descended from a terrestrial ancestor that eventually returned to the water.  Only independent evolution can explain this phenomenon.  According to Carroll, “When two species or groups of species evolve the same exact amino acids in a protein in adapting to similar environments, this is very strong evidence of natural selection for the same adaptation.”

But in some notable instances natural selection relaxes altogether, allowing harmful mutations to accumulate and to “fossilize” an organism’s DNA.  Take the coelacanth, for example, a large, primitive fish thought to be closely related to the first four-legged vertebrates.  With no MWS/LWS gene, the coelacanth’s only hope for color vision lies with its short-wavelength SWS gene.  But alas, this opsin, though still recognizable, is so riddled with mutations that it is no longer capable of constructing a functional protein.  Dolphins and whales also possess a fossilized SWS opsin gene.  But of course we shouldn’t feel sorry for these deep-water creatures because, unlike their ancestors, they have absolutely no use for color vision.  Predictably, nocturnal and subterranean mammals like the owl monkey, bush baby, slow loris, and blind mole rat also possess independently fossilized SWS genes.  These pathetically useless remnants of a primordial ancestor’s lifestyle supply solid evidence against theories of design.  Unlike an intelligent creator, “[n]atural selection cannot preserve what is not being used and it cannot plan for the future,” writes Carroll.  “The fossilization and loss of genes are exactly what is predicted to evolve in the absence of natural selection.”

At this point, one can hardly help but recognize a profound similarity in the mechanisms of vision among complex animals.  Indeed, recent discoveries have shown that tremendously different-looking eyes have much more in common than anyone had previously guessed.  For example, the same “tool kit” protein—now referred to as Pax-6—controls the construction of eyes belonging to creatures as diverse as worms, flies, mice, squid, and humans, implying an extremely ancient, common ancestor with a primitive eye composed of photoreceptor and pigment cells.  “The eye,” Carroll observes, “far from being one of the most difficult structures to account for by evolution, has become instead one of the leading sources of insights into how evolution works with common genetic tools to build complex organs.”

A remarkably comprehensive presentation, The Making of the Fittest does not, by any stretch of the imagination, confine itself to ocular-based discussions.  Readers will delight in challenging illuminations of everything from the evolution of human skin color and sickle cell anemia to the invention of an “antifreeze” gene in the bloodless icefish of Bouvet Island.  And despite two concluding chapters censuring the popular denial of science and ecological irresponsibility, Sean Carroll’s text is refreshingly void of pretension and politics—truly the work of a devoted scientist.

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One thought on “Book Review: Sean B. Carroll, The Making of the Fittest: DNA and the Ultimate Record of Evolution (NY: W.W. Norton 2006). 301 pp.

  1. E. Rafael

    Carroll never received the acclaim bestowed upon his physicist namesake. Too bad, because this Sean did some very nice work too. I have never read “Making.” (thanks for the review). But I did read “Endless Forms,” which was a true inspiration. I really look forward to more from Carroll.

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